Flow cytometric analysis of ATG5 expression in HepG2 cells using ATG5 antibody (R021346, 1:2,000). Green, isotype control; red, ATG5.

Immunocytochemical staining of HepG2 cells with ATG5 antibody (R021346, 1:1,000). Nuclei were stained blue with DAPI; ATG5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.

Western blotting analysis using ATG5 antibody (R021346). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATG5 antibody (R021346, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ATG5 antibody (R021346). ATG5 expression in wild type (WT) and ATG5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATG5 antibody (R021346, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.