Flow cytometric analysis of Phospholipase C gamma 1 expression in HepG2 cells using Phospholipase C gamma 1 antibody (R021339, 1:2,000). Green, isotype control; red, Phospholipase C gamma 1.

Immunocytochemical staining of HepG2 cells with Phospholipase C gamma 1 antibody (R021339, 1:1,000). Nuclei were stained blue with DAPI; Phospholipase C gamma 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using phospholipase C gamma 1 antibody (R021339). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospholipase C gamma 1 antibody (R021339, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using phospholipase C gamma 1 antibody (R021339). Phospholipase C gamma 1 expression in wild type (WT) and phospholipase C gamma 1 (PLCG1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospholipase C gamma 1 antibody (R021339, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.