Immunocytochemical staining of HepG2 cells using Alkylglycerone phosphate synthase antibody (R021284, 1:1,000), Top panel: wild-type (WT); Bottom panal: Alkylglycerone phosphate synthase shRNA knockdown (KD). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Alkylglycerone phosphate synthase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Alkylglycerone phosphate synthase antibody (R021284, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Alkylglycerone phosphate synthase expression in HeLa cells using Alkylglycerone phosphate synthase antibody (R021284, 1:2,000). Green, isotype control; red, Alkylglycerone phosphate synthase.

Immunocytochemical staining of Hela cells with Alkylglycerone phosphate synthase antibody (R021284, 1:1,000). Nuclei were stained blue with DAPI; Alkylglycerone phosphate synthase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using alkylglycerone phosphate synthase antibody (R021284). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with alkylglycerone phosphate synthase antibody (R021284, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using alkylglycerone phosphate synthase antibody (R021284). Alkylglycerone phosphate synthase expression in wild-type (WT) and alkylglycerone phosphate synthase (AGPS) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with alkylglycerone phosphate synthase antibody (R021284, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.