Flow cytometric analysis of Tribbles pseudokinase 3 expression in HT-1080 cells using Tribbles pseudokinase 3 antibody (R021268, 1:2,000). Green, isotype control; red, Tribbles pseudokinase 3.

Immunocytochemical staining of HT-1080 cells with Tribbles pseudokinase 3 antibody (R021268, 1:1,000). Nuclei were stained blue with DAPI; Tribbles pseudokinase 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar: 20 μm.

Western blotting analysis using Tribbles pseudokinase 3 antibody (R021268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Tribbles pseudokinase 3 antibody (R021268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Tribbles pseudokinase 3 antibody (R021268). Tribbles pseudokinase 3 expression in wild type (WT) and Tribbles pseudokinase 3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Tribbles pseudokinase 3 antibody (R021268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.