Flow cytometric analysis of Vasodilator stimulated phosphoprotein expression in HT-1080 cells using Vasodilator stimulated phosphoprotein antibody (R021261, 1:2,000). Green, isotype control; red, Vasodilator stimulated phosphoprotein.

Immunocytochemical staining of HT-1080 cells with Vasodilator stimulated phosphoprotein antibody (R021261, 1:1,000). Nuclei were stained blue with DAPI; Vasodilator stimulated phosphoprotein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Vasodilator stimulated phosphoprotein antibody (R021261). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Vasodilator stimulated phosphoprotein antibody (R021261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Vasodilator stimulated phosphoprotein antibody (R021261). Vasodilator stimulated phosphoprotein expression in wild type (WT) and Vasodilator stimulated phosphoprotein shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Vasodilator stimulated phosphoprotein antibody (R021261, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using Vasodilator stimulated phosphoprotein antibody (R021261, 1:1,000), Top panel: wild-type (WT); Bottom panal: Vasodilator stimulated phosphoprotein shRNA knockdown (KD). Nuclei were stained blue with DAPI; Vasodilator stimulated phosphoprotein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Vasodilator stimulated phosphoprotein knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Vasodilator stimulated phosphoprotein antibody (R021261, 1:2,000) and analyzed using BD flow cytometer.