Flow cytometric analysis of NFKB inhibitor alpha expression in HepG2 cells using NFKB inhibitor alpha antibody (R021233, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.

Immunocytochemical staining of HepG2 cells with NFKB inhibitor alpha antibody (R021233, 1:1,000). Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded mouse kidney using NFKB inhibitor alpha antibody (R021233, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded human prostatic adenocarcinoma using NFKB inhibitor alpha antibody (R021233, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using NFKB inhibitor alpha antibody (R021233). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NFKB inhibitor alpha antibody (R021233, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NFKB inhibitor alpha antibody (R021233). NFKB inhibitor alpha expression in wild-type (WT) and NFKB inhibitor alpha (NFKBIA) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NFKB inhibitor alpha antibody (R021233, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.