Flow cytometric analysis of DCP1A expression in HT-1080 cells using DCP1A antibody (R021190, 1:2,000). Green, isotype control; red, DCP1A.

Immunocytochemical staining of HT-1080 cells with DCP1A antibody (R021190, 1:1,000) . Nuclei were stained blue with DAPI; DCP1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using DCP1A antibody (R021190). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DCP1A antibody (R021190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using DCP1A antibody (R021190). DCP1A expression in wild type (WT) and DCP1A shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with DCP1A antibody (R021190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.