Immunohistochemistry was performed on paraffin-embedded mouse spleen using myocyte enhancer factor 2A antibody (R021162, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse brain using myocyte enhancer factor 2A antibody (R021162, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using myocyte enhancer factor 2A antibody (R021162). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with myocyte enhancer factor 2A antibody (R021162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using myocyte enhancer factor 2A antibody (R021162). Myocyte enhancer factor 2A expression in wild-type (WT) and myocyte enhancer factor 2A (MEF2A) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with myocyte enhancer factor 2A antibody (R021162, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.