Flow cytometric analysis of Golgi integral membrane protein 4 expression in HAP-1 cells using Golgi integral membrane protein 4 antibody (R021148, 1:2,000). Green, isotype control; red, Golgi integral membrane protein 4.

Immunocytochemical staining of HAP-1 cells with Golgi integral membrane protein 4 antibody (R021148, 1:1,000) . Nuclei were stained blue with DAPI; Golgi integral membrane protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm.

Western blotting analysis using Golgi integral membrane protein 4 antibody (R021148). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Golgi integral membrane protein 4 antibody (R021148, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Golgi integral membrane protein 4 antibody (R021148). Golgi integral membrane protein 4 expression in wild type (WT) and Golgi integral membrane protein 4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Golgi integral membrane protein 4 antibody (R021148, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Golgi integral membrane protein 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Golgi integral membrane protein 4 antibody (R021148, 1:2,000) and analyzed using BD flow cytometer.