Flow cytometric analysis of Glucose-6-phosphate isomerase expression in HeLa cells using Glucose-6-phosphate isomerase antibody (R021145, 1:2,000). Green, isotype control; red, Glucose-6-phosphate isomerase.

Immunocytochemical staining of Hela cells with Glucose-6-phosphate isomerase antibody (R021145, 1:1,000) . Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Glucose-6-phosphate isomerase antibody (R021145). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glucose-6-phosphate isomerase antibody (R021145, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Glucose 6 phosphate isomerase antibody (R021145). Glucose 6 phosphate isomerase expression in wild type (WT) and Glucose 6 phosphate isomerase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glucose 6 phosphate isomerase antibody (R021145, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Glucose-6-phosphate isomerase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glucose-6-phosphate isomerase antibody (R021145, 1:2,000) and analyzed using BD flow cytometer.