Flow cytometric analysis of GOPC expression in HeLa cells using GOPC antibody (R021130, 1:2,000). Green, isotype control; red, GOPC.

Immunocytochemical staining of Hela cells with GOPC antibody (R021130, 1:1,000). Nuclei were stained blue with DAPI; GOPC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using GOPC antibody (R021130). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GOPC antibody (R021130, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using GOPC antibody (R021130). GOPC expression in wild type (WT) and GOPC shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GOPC antibody (R021130, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.