Flow cytometric analysis of GTF2I  expression in HAP-1 cells using GTF2I  antibody (R021129, 1:2,000). Green, isotype control; red, GTF2I .

Immunocytochemical staining of HAP1 cells with GTF2I  antibody (R021129, 1:1,000) . Nuclei were stained blue with DAPI;GTF2I  was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using GTF2I  antibody (R021129). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GTF2I  antibody (R021129, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using GTF2I antibody (R021129). GTF2I expression in wild type (WT) and GTF2I shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GTF2I antibody (R021129, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.