Flow cytometric analysis of Growth factor receptor bound protein 2 expression in HT-1080 cells using Growth factor receptor bound protein 2 antibody (R021116, 1:2,000). Green, isotype control; red, Growth factor receptor bound protein 2.

Immunocytochemical staining of HT-1080 cells with Growth factor receptor bound protein 2 antibody (R021116, 1:1,000) . Nuclei were stained blue with DAPI; Growth factor receptor bound protein 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Growth factor receptor bound protein 2 antibody (R021116). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Growth factor receptor bound protein 2 antibody (R021116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Growth factor receptor bound protein 2 antibody (R021116). Growth factor receptor bound protein 2 expression in wild type (WT) and Growth factor receptor bound protein 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Growth factor receptor bound protein 2 antibody (R021116, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Growth factor receptor bound protein 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Growth factor receptor bound protein 2 antibody (R021116, 1:2,000) and analyzed using BD flow cytometer.