Flow cytometric analysis of Eukaryotic translation elongation factor 2 expression in HepG2 cells using Eukaryotic translation elongation factor 2 antibody (R021101, 1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 2.

Immunocytochemical staining of HepG2 cells with Eukaryotic translation elongation factor 2 antibody (R021101, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Eukaryotic translation elongation factor 2 antibody (R021101). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Eukaryotic translation elongation factor 2 antibody (R021101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Eukaryotic translation elongation factor 2 antibody (R021101). Eukaryotic translation elongation factor 2 expression in wild type (WT) and Eukaryotic translation elongation factor 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Eukaryotic translation elongation factor 2 antibody (R021101, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Eukaryotic translation elongation factor 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Eukaryotic translation elongation factor 2 antibody (R021101, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using EEF2 antibody (R021101, 1:1,000), Top panel: wild-type (WT); Bottom panal: EEF2 shRNA knockdown (KD). Nuclei were stained blue with DAPI;EEF2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.