Flow cytometric analysis of JNK3 expression in HT-1080 cells using JNK3 antibody (R021075, 1:2,000). Green, isotype control; red, JNK3.

Immunocytochemical staining of HT-1080 cells with JNK3 antibody (R021075, 1:1,000). Nuclei were stained blue with DAPI; JNK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using JNK3 antibody (R021075). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with JNK3 antibody (R021075, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using JNK3 antibody (R021075). JNK3 expression in wild type (WT) and JNK3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with JNK3 antibody (R021075, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.