[KD Validated] Anti-MTOR Rabbit mAb [77C89N02]

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[KD Validated] Anti-MTOR Rabbit mAb [77C89N02]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R021073
	20µL	¥738.00
	50µL	¥1380.00
	100µL	¥2280.00

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[KD Validated] Anti-MTOR Rabbit mAb [77C89N02]

Validation of mTOR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospho-mTOR (S2448) antibody (R021073, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Phospho-mTOR (S2448) expression in HeLa cells using Phospho-mTOR (S2448) antibody (R021073, 1:2,000). Green, isotype control; red, Phospho-mTOR (S2448).

Immunocytochemical staining of HeLa cells with Phospho-mTOR (S2448) antibody (R021073, 1:1,000). Nuclei were stained blue with DAPI; Phospho-mTOR (S2448) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Phospho-mTOR (S2448) antibody (R021073). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-mTOR (S2448) antibody (R021073, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Phospho-mTOR (S2448) antibody (R021073). Phospho-mTOR (S2448) expression in wild type (WT) and Phospho-mTOR (S2448) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Phospho-mTOR (S2448) antibody (R021073, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using Phospho-mTOR (S2448) antibody (R021073, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-mTOR (S2448) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-mTOR (S2448) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Product Name	[KD Validated] Anti-MTOR Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, FC, IF (Cell)/ICC
Dilution	WB 1:1,000~1:5,000; FC 1:200~1:2,000; IF 1:100~1:1,000
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	77C89N02
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Phospho-mTOR (S2448)
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Mechanistic Target Of Rapamycin Kinase; RAFT1; RAPT1; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FRAP1; FRAP2; FRAP; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Serine/Threonine-Protein Kinase MTOR; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FLJ44809; DJ576K7.1 (FK506 Binding Protein 12-Rapamycin Associated Protein 1); FK506 Binding Protein 12-Rapamycin Associated Protein 1; FKBP12-Rapamycin Complex-Associated Protein; Rapamycin Target Protein; EC 2.7.11.1; MTOR; SKS.
Molecular weight	Calculated MW: 289 kDa, Observed MW: 289 kDa
Uniprot ID	P42345
Gene ID	2475
Storage	Shipped on wet ice. Store at -20℃. Stable for 12 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	[KD Validated] Anti-MTOR Rabbit mAb [77C89N02] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	An atypical kinase belonging to the PIKK family of kinases. Controls cell growth through protein synthesis regulation. Downstream of PI3K/Akt pathway and required for cell survival. Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex.

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R021073 [KD Validated] Anti-MTOR Rabbit mAb [77C89N02]

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