Flow cytometric analysis of MARK2 expression in HT-1080 cells using MARK2 antibody (R021056, 1:2,000). Green, isotype control; red, MARK2.

Immunocytochemical staining of HT-1080 cells with MARK2 antibody (R021056, 1:1,000). Nuclei were stained blue with DAPI; MARK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using MARK2 antibody (R021056). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MARK2 antibody (R021056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using MARK2 antibody (R021056). MARK2 expression in wild type (WT) and MARK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MARK2 antibody (R021056, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.