Flow cytometric analysis of LYN expression in HepG2 cells using LYN antibody (R021053, 1:2,000). Green, isotype control; red, LYN.

Immunocytochemical staining of HepG2 cells with LYN antibody (R021053, 1:1,000). Nuclei were stained blue with DAPI; LYN was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using LYN antibody (R021053). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LYN antibody (R021053, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using LYN antibody (R021053). LYN expression in wild type (WT) and LYN shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LYN antibody (R021053, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.