Validation of NR2C2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with NR2C2 antibody (R021052, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of NR2C2 expression in HepG2 cells using NR2C2 antibody (R021052, 1:2,000). Green, isotype control; red, NR2C2.

Immunocytochemical staining of HepG2 cells with NR2C2 antibody (R021052, 1:1,000). Nuclei were stained blue with DAPI; NR2C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using NR2C2 antibody (R021052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NR2C2 antibody (R021052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NR2C2 antibody (R021052). NR2C2 expression in wild-type (WT) and NR2C2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NR2C2 antibody (R021052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.