Flow cytometric analysis of Protein arginine methyltransferase 5 expression in HeLa cells using Protein arginine methyltransferase 5 antibody (R021044, 1:2,000). Green, isotype control; red, Protein arginine methyltransferase 5.

Immunocytochemical staining of Hela cells with Protein arginine methyltransferase 5 antibody (R021044, 1:1,000). Nuclei were stained blue with DAPI; Protein arginine methyltransferase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Protein arginine methyltransferase 5 antibody (R021044). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Protein arginine methyltransferase 5 antibody (R021044, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using protein arginine methyltransferase 5 antibody (R021044). Protein arginine methyltransferase 5 expression in wild-type (WT) and protein arginine methyltransferase 5 (PRMT5) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with protein arginine methyltransferase 5 antibody (R021044, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.