Flow cytometric analysis of PIK3C3 expression in HeLa cells using PIK3C3 antibody (R021018, 1:2,000). Green, isotype control; red, PIK3C3.

Immunocytochemical staining of HeLa cells with PIK3C3 antibody (R021018, 1:1,000). Nuclei were stained blue with DAPI; PIK3C3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PIK3C3 antibody (R021018). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIK3C3 antibody (R021018, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PIK3C3 antibody (R021018). PIK3C3 expression in wild type (WT) and PIK3C3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PIK3C3 antibody (R021018, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.