Flow cytometric analysis of Pyruvate dehydrogenase kinase 1 expression in H9c2 cells using Pyruvate dehydrogenase kinase 1 antibody (R021015, 1:2,000). Green, isotype control; red, Pyruvate dehydrogenase kinase 1.

Immunocytochemical staining of H9C2 cells with Pyruvate dehydrogenase kinase 1 antibody (R021015, 1:1,000) . Nuclei were stained blue with DAPI; Pyruvate dehydrogenase kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain：Medium. Scale bar, 20 μm.

Western blotting analysis using Pyruvate dehydrogenase kinase 1 antibody (R021015). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyruvate dehydrogenase kinase 1 antibody (R021015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Pyruvate dehydrogenase kinsae 1 antibody (R021015). Pyruvate dehydrogenase kinsae 1 expression in wild type (WT) and Pyruvate dehydrogenase kinsae 1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Pyruvate dehydrogenase kinsae 1 antibody (R021015, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.