Immunocytochemical staining of H9c2 cells with Phospho-PLCB3 (S537) antibody (R021007, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-PLCB3 (S537) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-PLCB3 (S537) antibody (R021007). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-PLCB3 (S537) antibody (R021007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using phospho-PLCB3 (S537) antibody (R021007). Phospho-PLCB3 (S537) expression in wild-type (WT) and PLCB3 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PLCB3 (S537) antibody (R021007, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.