Flow cytometric analysis of PRKCSH expression in Hela cells using PRKCSH antibody (R021001, 1:2,000). Green, isotype control; red, PRKCSH.

Immunocytochemical staining of Hela cells with PRKCSH antibody (R021001, 1:1,000) . Nuclei were stained blue with DAPI; PRKCSH was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PRKCSH antibody (R021001). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKCSH antibody (R021001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PRKCSH antibody (R021001. PRKCSH expression in wild type (WT) and PRKCSH shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PRKCSH antibody (R021001, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using PRKCSH antibody (R021001, 1:1,000), Top panel: wild-type (WT); Bottom panal: PRKCSH shRNA knockdown (KD). Nuclei were stained blue with DAPI; PRKCSH was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.