Flow cytometric analysis of Pyrophosphatase 1 expression in HT-1080 cells using Pyrophosphatase 1 antibody (R020998, 1:2,000). Green, isotype control; red, Pyrophosphatase 1.

Immunocytochemical staining of HT-1080 cells with Pyrophosphatase 1 antibody(R020998, 1:1,000) . Nuclei were stained blue with DAPI; Pyrophosphatase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Pyrophosphatase 1 antibody (R020998). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Pyrophosphatase 1 antibody (R020998, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Pyrophosphatase 1 antibody (R020998). Pyrophosphatase 1 expression in wild type (WT) and Pyrophosphatase 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Pyrophosphatase 1 antibody (R020998, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.