Flow cytometric analysis of RAD21 expression in HepG2 cells using RAD21 antibody (R020996, 1:2,000). Green, isotype control; red, RAD21.

Immunocytochemical staining of HepG2 cells with RAD21 antibody (R020996, 1:1,000). Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using RAD21 antibody (R020996). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RAD21 antibody (R020996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using RAD21 antibody (R020996). RAD21 expression in wild type (WT) and RAD21 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with RAD21 antibody (R020996, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of RAD21 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with RAD21 antibody (R020996, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using RAD21 antibody (R020996, 1:1,000), Top panel: wild-type (WT); Bottom panal: RAD21 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.