Flow cytometric analysis of Forkhead box O4 expression in HeLa cells using Forkhead box O4 antibody (R020988, 1:2,000). Green, isotype control; red, Forkhead box O4.

Immunocytochemical staining of HeLa cells with Forkhead box O4 antibody (R020988, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Forkhead box O4 antibody (R020988). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Forkhead box O4 antibody (R020988, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Forkhead box O4 antibody (R020988). Forkhead box O4 expression in wild type (WT) and Forkhead box O4 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Forkhead box O4 antibody (R020988, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of 293T cells using Forkhead box O4 antibody (R020988, 1:1,000), Top panel: wild-type (WT); Bottom panal: Forkhead box O4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Forkhead box O4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Western blotting analysis using Forkhead box O4 antibody (R020988).Forkhead box O4 expression in wild type (WT) and FOXO4 knockout (KO) 293T cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Forkhead box O4 antibody (R020988, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.