Validation of SMARCA4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SMARCA4 antibody (R020967, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of SMARCA4 expression in HepG2 cells using SMARCA4 antibody (R020967, 1:2,000). Green, isotype control; red, SMARCA4.

Immunocytochemical staining of HepG2 cells with SMARCA4 antibody (R020967, 1:1,000). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using SMARCA4 antibody (R020967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMARCA4 antibody (R020967, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using SMARCA4 antibody (R020967). SMARCA4 expression in wild type (WT) and SMARCA4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMARCA4 antibody (R020967, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of Hela cells using SMARCA4 antibody (R020967, 1:1,000), Top panel: wild-type (WT); Bottom panal: SMARCA4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.