Validation of Vascular endothelial growth factor D knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Vascular endothelial growth factor D antibody (R020960, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Vascular endothelial growth factor D expression in HepG2 cells using Vascular endothelial growth factor D antibody (R020960, 1:2,000). Green, isotype control; red, Vascular endothelial growth factor D.

Immunocytochemical staining of HepG2 cells with Vascular endothelial growth factor D antibody (R020960, 1:1,000). Nuclei were stained blue with DAPI; Vascular endothelial growth factor D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Vascular endothelial growth factor D antibody (R020960). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Vascular endothelial growth factor D antibody (R020960, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using anti-Vascular endothelial growth factor D antibody (R020960). Vascular endothelial growth factor D expression in wild type (WT) and Vascular endothelial growth factor D shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Vascular endothelial growth factor D antibody (R020960, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.