Validation of transcription factor Dp-1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with transcription factor Dp-1 antibody (R020906, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of transcription factor Dp-1 expression in C2C12 cells using transcription factor Dp-1 antibody (R020906, 1:2,000). Green, isotype control; red, transcription factor Dp-1.

Immunocytochemical staining of C2C12 cells with Transcription factor Dp-1 antibody (R020906, 1:1,000) . Nuclei were stained blue with DAPI; Transcription factor Dp-1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using transcription factor Dp-1 antibody (R020906). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with transcription factor Dp-1 antibody (R020906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using transcription factor Dp-1 antibody (R020906). Transcription factor Dp-1 expression in wild-type (WT) and transcription factor Dp-1 (TFDP1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with transcription factor Dp-1 antibody (R020906, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of Hela cells using Transcription factor Dp-1 antibody (R020906, 1:1,000), Top panel: wild-type (WT); Bottom panal:Transcription factor Dp-1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;Transcription factor Dp-1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.