Flow cytometric analysis of Mitogen-activated protein kinase 1 expression in C2C12 cells using Mitogen-activated protein kinase 1 antibody (R020882, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 1.

Immunocytochemical staining of C2C12 cells with Mitogen-activated protein kinase 1 antibody (R020882, 1:1,000) . Nuclei were stained blue with DAPI; Mitogen-activated protein kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using leica stellaris 5. Protein abundance based on laser intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Mitogen-activated protein kinase 1 antibody (R020882). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Mitogen-activated protein kinase 1 antibody (R020882, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using mitogen-activated protein kinase 1 antibody (R020882). Mitogen-activated protein kinase 1 expression in wild-type (WT) and mitogen-activated protein kinase 1 (MAPK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 1 antibody (R020882, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Mitogen-activated protein kinase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Mitogen-activated protein kinase 1 antibody (R020882, 1:2,000) and analyzed using BD flow cytometer.