Immunocytochemical staining of HepG2 cells with phospho-cyclin B1 (Ser126) antibody (R020868, 1:1,000). Nuclei were stained blue with DAPI; Phospho-cyclin B1 (Ser126) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using phospho-cyclin B1 (Ser126) antibody (R020868). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-cyclin B1 (Ser126) antibody (R020868, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using phospho-cyclin B1 (Ser126) antibody (R020868). Phospho-cyclin B1 (Ser126) expression in wild type (WT) and CCNB1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-cyclin B1 (Ser126) antibody (R020868, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HT-1080 cells using Phospho-Cyclin B1 (Ser126) antibody (R020868, 1:1,000), Top panel: wild-type (WT); Bottom panal: Phospho-Cyclin B1 (Ser126) shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospho-Cyclin B1 (Ser126) was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.