Flow cytometric analysis of cyclin dependent kinase 2 expression in HepG2 cells using cyclin dependent kinase 2 antibody (R020867, 1:2,000). Green, isotype control; red, cyclin dependent kinase 2.

Immunocytochemical staining of HepG2 cells with Cyclin dependent kinase 2 antibody(R020867, 1:1,000) . Nuclei were stained blue with DAPI; Cyclin dependent kinase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using cyclin dependent kinase 2 antibody (R020867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cyclin dependent kinase 2 antibody (R020867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using cyclin dependent kinase 2 antibody (R020867). Cyclin dependent kinase 2 expression in wild type (WT) and cyclin dependent kinase 2 (CDK2) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with cyclin dependent kinase 2 antibody (R020867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Cyclin dependent kinase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Cyclin dependent kinase 2 antibody (R020867, 1:2,000) and analyzed using BD flow cytometer.