Immunocytochemical staining of HeLa cells using EIF4EBP1 antibody (R020859, 1:1,000), Top panel: wild-type (WT); Bottom panal: EIF4EBP1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of EIF4EBP1 expression in C2C12 cells using EIF4EBP1 antibody (R020859, 1:2,000). Green, isotype control; red, EIF4EBP1.

Immunocytochemical staining of C2C12 cells with EIF4EBP1 antibody (R020859, 1:1,000) . Nuclei were stained blue with DAPI; EIF4EBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.

Western blotting analysis using EIF4EBP1 antibody (R020859). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with EIF4EBP1 antibody (R020859, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit. EIF4EBP1, eukaryotic translation initiation factor 4E binding protein 1.

Western blotting analysis using EIF4EBP1 antibody (R020859). EIF4EBP1 expression in wild-type (WT) and EIF4EBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with EIF4EBP1 antibody (R020859, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.