Flow cytometric analysis of HDAC4 expression in HepG2 cells using HDAC4 antibody (R020827, 1:2,000). Green, isotype control; red, HDAC4.

Immunocytochemical staining of HepG2 cells with HDAC4 antibody (R020827, 1:1,000). Nuclei were stained blue with DAPI; HDAC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using HDAC4 antibody (R020827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HDAC4 antibody (R020827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using HDAC4 antibody (R020827). HDAC4 expression in wild type (WT) and HDAC4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HDAC4 antibody (R020827, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.