Immunocytochemical staining of HT-1080 cells with BCL2 like 1 antibody(R020793, 1:1,000) . Nuclei were stained blue with DAPI; BCL2 like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using BCL2 like 1 antibody (R020793). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 1 antibody (R020793, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BCL2 like 1 antibody (R020793). BCL2 like 1 expression in wild type (WT) and BCL2 like 1 (BCL2L1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BCL2 like 1 antibody (R020793, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of BCL2 like 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with BCL2 like 1 antibody (R020793, 1:2,000) and analyzed using BD flow cytometer.