Flow cytometric analysis of ALPP expression in HepG2 cells using ALPP antibody (R020787, 1:2,000). Green, isotype control; red, ALPP.

Immunocytochemical staining of HepG2 cells with ALPP antibody (R020787, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using ALPP antibody (R020787). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ALPP antibody (R020787, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ALPP antibody (R020787). ALPP expression in wild type (WT) and ALPP shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ALPP antibody (R020787, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.