Validation of G protein subunit beta 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with G protein subunit beta 2 antibody (R020740, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of G protein subunit beta 2 expression in HT-1080 cells using G protein subunit beta 2 antibody (R020740, 1:2,000). Green, isotype control; red, G protein subunit beta 2.

Immunocytochemical staining of HT-1080 cells with G protein subunit beta 2 antibody (R020740, 1:1,000) . Nuclei were stained blue with DAPI; G protein subunit beta 2n was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using G protein subunit beta 2 antibody (R020740). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein subunit beta 2 antibody (R020740, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using G protein subunit beta 2 antibody (R020740). G protein subunit beta 2 expression in wild type (WT) and G protein subunit beta 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with G protein subunit beta 2 antibody (R020740, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.