Flow cytometric analysis of GNAQ expression in C2C12 cells using GNAQ antibody (R020712, 1:2,000). Green, isotype control; red, GNAQ.

Immunocytochemical staining of C2C12 cells with G protein subunit alpha q antibody (R020712, 1:1,000). Nuclei were stained blue with DAPI; G protein subunit alpha q was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using G protein subunit alpha q antibody (R020712). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with G protein subunit alpha q antibody (R020712, 1;5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using G protein subunit alpha q antibody (R020712). G protein subunit alpha q expression in wild type (WT) and G protein subunit alpha q shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with G protein subunit alpha q antibody (R020712, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.