Flow cytometric analysis of BCR expression in HepG2 cells using BCR antibody (R020689, 1:2,000). Green, isotype control; red, BCR.

Immunocytochemical staining of HepG2 cells with BCR antibody (R020689, 1:1,000) . Nuclei were stained blue with DAPI; BCR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using BCR antibody (R020689). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCR antibody (R020689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BCR antibody (R020689). BCR expression in wild-type (WT) and BCR shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCR antibody (R020689, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of BCR knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with BCR antibody (R020689, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using BCR antibody (R020689, 1:1,000), Top panel: wild-type (WT); Bottom panal: BCR shRNA knockdown (KD). Nuclei were stained blue with DAPI; was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.