Flow cytometric analysis of PCAF expression in HT-1080 cells using PCAF antibody (R020622, 1:2,000). Green, isotype control; red, PCAF.

Immunocytochemical staining of HT-1080 cells with PCAF antibody (R020622, 1:1,000). Nuclei were stained blue with DAPI; PCAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PCAF antibody (R020622). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PCAF antibody (R020622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PCAF antibody (R020622).PCAF expression in wild type (WT) and PCAF shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PCAF antibody (R020622, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.