Immunocytochemical staining of HT-1080 cells using PI3 Kinase p110 beta antibody (R020620, 1:1,000), Top panel: wild-type (WT); Bottom panal: PI3 Kinase p110 beta shRNA knockdown (KD). Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of PI3 Kinase p110 beta expression in HT-1080 cells using PI3 Kinase p110 beta antibody (R020620, 1:2,000). Green, isotype control; red, PI3 Kinase p110 beta.

Immunocytochemical staining of HepG2 cells with PI3 Kinase p110 beta antibody (R020620, 1:1,000) . Nuclei were stained blue with DAPI; PI3 Kinase p110 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using PI3 Kinase p110 beta antibody (R020620). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p110 beta antibody (R020620, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PI3 Kinase p110 beta antibody (R020620). PI3 Kinase p110 beta expression in wild type (WT) and PI3 Kinase p110 beta shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with PI3 Kinase p110 beta antibody (R020620, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of PI3 Kinase p110 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with PI3 Kinase p110 beta antibody (R020620, 1:2,000) and analyzed using BD flow cytometer.