Flow cytometric analysis of STK4 expression in HepG2 cells using STK4 antibody (R020475, 1:2,000). Green, isotype control; red, STK4.

Western blotting analysis using STK4 antibody (R020475). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STK4 antibody (R020475, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using STK4 antibody (R020475). STK4 expression in wild-type (WT) and STK4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STK4 antibody (R020475, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of STK4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with STK4 antibody (R020475, 1:2,000) and analyzed using BD flow cytometer.