Western blotting analysis using KRIT1 antibody (R020406). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KRIT1 antibody (R020406, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using KRIT1 antibody (R020406). KRIT1 expression in wild-type (WT) and KRIT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KRIT1 antibody (R020406, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of KRIT1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with KRIT1 antibody (R020406, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of C2C12 cells with KRIT1 antibody (R020406, 1:1,000) . Nuclei were stained blue with DAPI; KRIT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.