Flow cytometric analysis of TEAD4 expression in HepG2 cells using TEAD4 antibody (R020389, 1:2,000). Green, isotype control; red, TEAD4.

Western blotting analysis using TEAD4 antibody (R020389). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TEAD4 antibody (R020389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using TEAD4 antibody (R020389). TEAD4 expression in wild type (WT) and TEAD4 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TEAD4 antibody (R020389, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HepG2 cells with TEAD4 antibody (R020389, 1:1,000) . Nuclei were stained blue with DAPI; TEAD4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.