Flow cytometric analysis of Cyclin A2 expression in HepG2 cells using Cyclin A2 antibody (R020334, 1:2,000). Green, isotype control; red, Cyclin A2.

Immunocytochemical staining of HepG2 cells with Cyclin A2 antibody (R020334, 1:1,000). Nuclei were stained blue with DAPI; Cyclin A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using Cyclin A2 antibody (R020334). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin A2 antibody (R020334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Cyclin A2 antibody (R020334). Cyclin A2 expression in wild type (WT) and Cyclin A2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Cyclin A2 antibody (R020334, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of CyclinA2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CyclinA2 antibody (R020334, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Cyclin A2 antibody (R020334, 1:1,000), Top panel: wild-type (WT); Bottom panal: Cyclin A2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Cyclin A2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.