Flow cytometric analysis of Glycogen synthase kinase 3 beta expression in HepG2 cells using Glycogen synthase kinase 3 beta antibody (R020322, 1:2,000). Green, isotype control; red, Glycogen synthase kinase 3 beta.

Validation of Glycogen synthase kinase 3 beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glycogen synthase kinase 3 beta antibody (R020322, 1:2,000) and analyzed using BD flow cytometer.

Immunocytochemical staining of HT-1080 cells with Glycogen synthase kinase 3 beta antibody (R020322, 1:1,000). Nuclei were stained blue with DAPI; Glycogen synthase kinase 3 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using glycogen synthase kinase 3 beta antibody (R020322, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using Glycogen synthase kinase 3 beta antibody (R020322). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glycogen synthase kinase 3 beta antibody (R020322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Glycogen synthase kinase 3 beta antibody (R020322). Glycogen synthase kinase 3 beta expression in wild type (WT) and glycogen synthase kinase 3 beta shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Glycogen synthase kinase 3 beta antibody (R020322, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.