Flow cytometric analysis of ATP citrate lyase expression in HepG2 cells using ATP citrate lyase antibody (R020320, 1:2,000). Green, isotype control; red, ATP citrate lyase.

Immunocytochemical staining of HepG2 cells with ATP citrate lyase antibody (R020320, 1:1,000). Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using ATP citrate lyase antibody (R020320). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP citrate lyase antibody (R020320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using ATP citrate lyase antibody (R020320). ATP citrate lyase expression in wild type (WT) and ATP citrate lyase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with ATP citrate lyase antibody (R020320, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using ATP citrate lyase antibody (R020320, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP citrate lyase shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.