Flow cytometric analysis of PRAS40 expression in C2C12 cells using PRAS40 antibody (R020311, 1:2,000). Green, isotype control; red, PRAS40.

Immunocytochemical staining of C2C12 cells with PRAS40 antibody (R020311, 1:1,000). Nuclei were stained blue with DAPI; PRAS40 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using PRAS40 antibody (R020311). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRAS40 antibody (R020311, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using PRAS40 antibody (R020311). PRAS40 expression in wild type (WT) and PRAS40 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRAS40 antibody (R020311, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.