Validation of Daxx knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Daxx antibody (R020285, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Daxx expression in HepG2 cells using Daxx antibody (R020285, 1:2,000). Green, isotype control; red, Daxx.

Immunocytochemical staining of HepG2 cells with Daxx antibody (R020285, 1:1,000). Nuclei were stained blue with DAPI; Daxx was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Daxx antibody (R020285). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Daxx antibody (R020285, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Daxx antibody (R020285). Daxx expression in wild type (WT) and Daxx shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Daxx antibody (R020285, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.